FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61
Sequences flagged as poor quality0
Sequence length116-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATCCTTTGCAGATGTAGTTGTTTCTGCTACAGAAGAATCGGTCTTT11.639344262295082No Hit
GTCGACAAGCTTCATGAATAATTAAGTACATTTAGTTAAAACATTTTTCA11.639344262295082No Hit
GGACGAATAGCGGACTTTTTAAAGGCTTTACTAAAGAAGAGTTTGTTTAT11.639344262295082No Hit
CATTGATGCAGATAAGCATATTCATCGGAATTCACCGGATAGATCTCCAG11.639344262295082No Hit
GCAGCAATTTCTACCGGTTGCAAGGTAGCTAATCCGAAACTCACCGTGTG11.639344262295082No Hit
ATCAAATATAAACATATCCACGTCTCGCAAAAAGAGTCTAGCAAGAATGA11.639344262295082No Hit
TGACCATAACCAGTATGGTCTGGGCGGTGATATTTGGTATGGCGGCGGGA11.639344262295082No Hit
CTTCTATCTGGAGCCCGTGGGTGGTATCATTTCTCTCCGGTGGATAGATA11.639344262295082No Hit
CCGAAAGGAGCAAGTATCTTTTATGAACCGCCAAAATCATTCTGAGCCGG11.639344262295082No Hit
GTCCATGTGGGACAGCACCGCATAGGCGGAATGGAGAGAAACTTCGTACT11.639344262295082No Hit
GTCATGAAAATACACAAGGCTTGTGATTACATTAGTTTGGATTGGCTTCT11.639344262295082No Hit
CTTCTAAAAGACAGTTGCAGAGTGGTTTAGCACTGTGTATATGTAAGCTT11.639344262295082No Hit
CACTGATTAGCGATGCTGTGAGTTCGCTGTGACGCCCTGAGCTCTCTGAC11.639344262295082No Hit
AGCGGCATTGACCGCAAGTGGGAAGAATTGTCCAGGTTGCTTCAAGATAA11.639344262295082No Hit
ACTGCATCTAAAATGGACTCAGTAAAAAGGCAAAACAGCTCAGAAGATGA11.639344262295082No Hit
CAGCGGTGATGGCATGGCTGAAAAAGCGGATTGCGGTTCAGCTAAGGCTG11.639344262295082No Hit
CTTTGCGTTTTGGTAGCAAAAGTGAAGGTTTTGGTATTACTTAGGTACGA11.639344262295082No Hit
TCGATGTACTCTTTTACCTTATTCTCCTTGCCTCCGATCGTACGAACTAC11.639344262295082No Hit
TGATGCTTACCACATGGCTGGGTAAGAAAGGGTTTGTCGATTTATCCACA11.639344262295082No Hit
GCAATCCCGAAAATTGCCATCAGGCAAATTCCATTTCCCGCATACTCTGC11.639344262295082No Hit
CCGGATTTCACCTCGGATAGTGCTTCTGTCATCGCCTCTTCATTCTGCTC11.639344262295082No Hit
CTCACAATCAGATAATCCATATGATTCTAAAAATCTATTAAGATAACAGT11.639344262295082No Hit
GGCAGCCTGGCGTTGGGAGCGACCAAATTGCAAACTATCCGCTACGTGAT11.639344262295082No Hit
CGGATGTGTAATTATATTATTTTCTGTGATACCGTCCAAAAATGCACCGG11.639344262295082No Hit
TACAACTGCTGCTGAAGCTATTGCTGATAATGAACATGTTTGCAAGATTG11.639344262295082No Hit
CATTGCTTCCTCAATAGTTGAAATATTGTCAATGTAAGTGCCCATGCTGT11.639344262295082No Hit
CTTCAAGAAGCTGGGACCGAAATTCGGTAAGCAGATGAAAGCCGTGGCTG11.639344262295082No Hit
GGCAAGGGAAAAATCTGCTGTATCGTTCCTGCACACAATGCCCAAAATCT11.639344262295082No Hit
TTTAAACCATTAAATGCAAAACTAACAGCTTTACCACCTAGAAGTTTAGC11.639344262295082No Hit
CCTTCGTCGCCTTGAATGCCGTGGCTATGACTCCTGCGGGGTCGCCACGC11.639344262295082No Hit
CAACAAGACGCCGCCGGTGGCCATGCTTATGTATTGTTGTGTGCTTATCA11.639344262295082No Hit
GACAGGCTGACCCGCCGATTCGGAACGGGTGCGGTCACCGCCGCCAGCGT11.639344262295082No Hit
CACAACCACGACCTGTTGGGAGAACGAAATAGGAAAACGCATAAGGAAAA11.639344262295082No Hit
TTCTTCTCGGCTTTTTCTACGCTGGCTATGCTGCCTCTGGCCATGCTGGA11.639344262295082No Hit
ATATAGTACACGTTATTGTCTATTCCCTTGAAGGAGAAGCACGGTCCCAT11.639344262295082No Hit
CCAAAGTTGGAGGCTACCGGGTGTTCCTCCGATAAATCCTGTGCCGACAG11.639344262295082No Hit
AAACGTTTATGATTGCTATGGCAAACGAAGGGTATGACACTTGTCCTCTG11.639344262295082No Hit
AATATTATCAGTTCATTCTATAGAAAATTTGAATAATGATGAAGAATATT11.639344262295082No Hit
AAGTGACGCAGTTTTCCAAGTCCTTTTTCTTCCAGCAGCGCGTTGGCTTC11.639344262295082No Hit
GCGCCACCATGGTATTTTCGCTGCTGGCGTTCAGCGCCGCCAGCGTAAGC11.639344262295082No Hit
GATCGAACGCGACAACATCAATCCCGCTTACAAAATCATGTGGCTTCTCA11.639344262295082No Hit
CCATGGCGTCCACGTCGGTGGCCGGGATGGCCTTGCAGTGGGCGTCATAG11.639344262295082No Hit
CTCCTTGATCATCTGCGCCGGTTCATTCTTGGTATGGTTGGTGATCATCC11.639344262295082No Hit
ATGGAAGATAGGCACACCGGGGAACATGGCGAACGATGAACACACCAGGA11.639344262295082No Hit
TGCCTGCCTGAATAAACCAAAGGGGTATTTGTACGCATGACTTCGCGACC11.639344262295082No Hit
CATAAGAGAAAAAGTGGGACTTGTACTTCAAAATCCCGACAACCAGCTTA11.639344262295082No Hit
GTGTGCCTGAGGTCAGCGTCACGCTGGACAGCGGCGACACCATCCGCATC11.639344262295082No Hit
GCCTTGTAGTCGCTGTGCCCCGTCCATTTCATCACCACTTGTGCGGGAAT11.639344262295082No Hit
CCTTCCGGTGCAGTCTTTTCTACTACATAGTAAGAACCAAGTGGCAGGTT11.639344262295082No Hit
GAACTTTATAAAACGCTCAATGATGCGCAAAAACAGGAAGTCTGTAACAT11.639344262295082No Hit
GATCGCGTTGATCTCGCCGCCCAGCAGCACGACGTGCGTCTCCGGATACC11.639344262295082No Hit
AGATGTTCCAGGTAACGACTCTGGACATGAACAATGTGCCTAAGACCGAG11.639344262295082No Hit
ATGTACTTGGAACAGCGTGCGGAGGAAGATGCGCTATTTGCAAAGAAGTA11.639344262295082No Hit
GTTATGGAATGCAGTGCAAATCGCAGGGGCGAGGCATGGTGCAAATGGAA11.639344262295082No Hit
CGCCCCTCTGGTCATTTCCATTGACCGGGAATACGGTTCCGGCGGCCATG11.639344262295082No Hit
AAGAGCGATCATATTGAAAATGGTCATCAGGCCGATGCCCACATCGCCCA11.639344262295082No Hit
CTCAATTTCATCAACTGGCTTTTCGGTAGTCTGTAAATATTGTGCCCGCG11.639344262295082No Hit
GGCTGGCCCCAATCCTGGCCCTGCTGGTTATAGAAGTCCGGCGGGCAGCC11.639344262295082No Hit
TGTCATCCCATAAACGATACTGATTCCGTTTGTCTGCTCTTCATCGTCAA11.639344262295082No Hit
CGGCACAATTGCAATTATATATATGTATCGTTTCCGCGATCTGAGTTATG11.639344262295082No Hit
TGTAAATCCACCCATGCCTTGCTTCCAACTAATTTTGTTCCTTATACTCA11.639344262295082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGA50.0145.04
AATCCCG50.0145.03
GAAAATT50.0145.09
CAATCCC50.0145.02
GCAATCC50.0145.01
CCCGAAA50.0145.06
CGAAAAT50.0145.08
TCCCGAA50.0145.05
TCCGATA50.0145.0145
CCGAAAA50.0145.07
Produced by FastQC (version 0.11.5)