FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909996
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCT2800.003240700815.3345058
CACAAAT7050.00145613869.2241481
ATCAAAC8054.8582425E-48.9758791
CTCCTGA6650.0093613378.692431
CTTTTAA9452.5657116E-48.4107311
CTCAAAC7850.00386083828.2841071
CTTTTCG10205.834152E-47.7922951
TCAAGAT11603.1190526E-47.4040842
CAGGCTT9950.0043246847.19206248
GAATATT12951.4034931E-47.1849164
AACAATG11200.00174611127.02947332
CCTTGAG10400.0060510396.9476751
AGAAGGT13500.00175551266.36202863
CAACAAT12550.00521710846.33317951
CTTTCTT23256.787195E-76.21555471
CTCGCCG14050.00247154646.1713161
CAAGATG14350.0034815315.9851833
CTCAAAG14950.0049413845.79979851
AAGACAA14950.0054905235.7449753
ATCAAGA16750.0028167835.6079151
Produced by FastQC (version 0.11.5)