FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444152
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTT4400.004083825711.6373891
CTCACAT5800.00274411810.089561
CTTAAAT12205.6999852E-89.5933511
CCCAGCT10659.302212E-68.9290231
CTCGTAT6750.009570988.6695481
CTCAATC9452.248932E-48.5147331
CCCGTGG7800.00326392368.4403051
CTATAAC9150.00153480327.99445961
GTTCGGT10506.9824804E-47.6632611
TAATCTC11502.5542927E-47.534379
CACAGAT12758.858512E-57.4583611
CCTCAAT10300.0048937637.10187441
CCCAAAT11500.00183578836.99688961
CTTTGAA23059.03492E-96.98171231
CTTGGAT10500.00589851666.9666011
CTTGATC10550.0061765326.93358371
CTCTTTT32750.06.92405651
CTTTAAG11700.00220204736.8772851
CTCATAG10700.0070812796.83638331
ATCGACC10600.0073306996.811972
Produced by FastQC (version 0.11.5)