FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805987
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2859.685491E-619.6309787
ACACGTC2951.3042227E-518.965528
CACGTCT3001.50751985E-518.649439
GCACACG3709.0907895E-515.1230346
CTCAGAT4702.7540646E-614.9011261
AGAGCAC4102.1757906E-413.6476163
CTCCAAG5801.65446E-613.2825551
AGCACAC4757.5286656E-411.7800475
CCCATGA5501.9723516E-411.460321
GAGCACA5000.00115700411.1910464
CTCTAAT4400.005645291411.1419771
CCCAGAA6305.613479E-511.1167131
CCCAGAT4600.00785580810.6575441
ATCACGA5450.002347010710.280411
CTTCTAA7603.3488286E-510.1366871
ATCGAAC5600.00297185579.9920052
CTTATGT6357.43924E-49.9262621
CCCAGCT5700.00340393659.8295141
CTTTAGA7905.1239575E-59.7517491
CTATTGA8056.2966275E-59.570041
Produced by FastQC (version 0.11.5)