FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870356
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA7350.00189543178.9608481
CTTAAAG7850.003441898.3900931
CTTATAT12901.0168623E-47.37475441
GAACCAG10200.00501162147.0838285
CTCAAAA12656.52633E-46.9420011
TGAATTT17852.8988761E-66.88143253
CAAAGAA22755.1142706E-86.7551011
GTTCTGC12859.0197736E-46.74755246
CTCTTTT20102.1587257E-66.55345541
CTTCCGG15106.9821085E-46.3002871
CTTCCTT16402.7820075E-46.24709561
CTTCAAT12900.0060843036.2401771
CTTCAAA13100.0071377096.1449071
AATGCCA13000.0075267536.1135377
CTCCTGC13250.0080310416.0753421
CCTCTTT14900.00413272955.8937121
GATTTTG19103.723472E-45.674485
CTTCTTT25951.304372E-45.0760871
Produced by FastQC (version 0.11.5)