FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850694
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3356.385562E-821.337089
CACACGT3151.9015417E-518.1534527
ACACGTC3653.4162185E-617.6250158
AGCACAC5009.6488086E-411.4366755
CTACAAT6809.6384436E-510.54077151
AGAGCAC5850.0035612629.7760863
GTTTGTA6900.00128757529.3492061
CTCGGCT6500.0082941218.8218151
AAGAGCA11150.00168412287.053422
AGATGCT10450.0070250856.8413244
CATTGAT11550.00237839186.82640461
CTTCCAG13702.7515372E-46.8014911
GTTTCAT11950.00340077426.59790561
TTATTCA11950.00349434026.58083774
Produced by FastQC (version 0.11.5)