FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995889
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGTA3558.127039E-414.4226481
TTCGATC4800.00857487210.5328412
CTCAACG6758.7517884E-49.7524591
CTTCACT9251.7215216E-59.4888781
TTAGATA7703.1275285E-49.3798952
TCGATCT7904.0374717E-49.1438063
CAATTGA7300.001789969.0176841
CTCATGC6500.0070269479.0022691
CATAGAG7350.00190463728.9563381
CTTCAAA18004.285903E-87.72069641
CTTCGTT10658.124749E-47.55472141
CTATAAT13201.3598386E-47.20352031
CTCTTAT10250.00466812037.1359451
GTTTATT14555.8848236E-57.03785661
CTCTTCA13852.455026E-46.8654491
CAAGAAC13902.5657934E-46.84075261
CTCAAAG10800.00774857176.77254061
CTCTTTT24956.146365E-96.74268151
CTTCATT18504.193822E-66.72128871
TGATCTG12000.00327657026.621644
Produced by FastQC (version 0.11.5)