FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129474
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3150.006753206313.7297247
TCAGAGT4550.005848193511.0898852
CTCAGAT6959.426481E-510.5645811
CTCAAAT9351.6882623E-610.2086631
CCCCATT7502.035086E-49.7898451
GTTTAAA7602.3252887E-49.6610321
CTTTAAT12205.3678814E-68.4256861
CTCAATG10104.4221012E-47.99665551
CATCGAT8700.008417617.59556961
CTTAAAA13602.2932418E-57.55833631
CCCGTTT10859.5136644E-47.4438911
CCCAGAA10700.006826386.86204051
CTTCAGG12050.00288510726.7025911
CTTCTAT11050.0093149896.6446911
AACTTTA10900.00975514956.6129414
TTTTACG12550.0053532276.3178617
CTTTTAT19951.2771368E-56.25666761
CTTCAAA17751.1014601E-46.20483161
CTTTTGT19104.5414105E-56.15068861
ATATCCC13000.00771736.09916646
Produced by FastQC (version 0.11.5)