FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665824
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAC2850.003151210715.3981991
CCCTCGT4450.00444252511.5053961
CTCAAAG6807.844542E-510.7560951
CTCCGCT5500.001764302610.6387551
CAATGAA7800.00326229548.4393971
CTCCTTT11400.001672397.0575081
CAACAAA10400.00537186267.03283071
ATATCCG11500.00209734366.9078396
CTTTCAT10750.00740299846.80385541