FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443028
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAC4900.00911569310.4467821
GTAAGAC7000.00122397169.4021041
CCCCATT7350.00190951648.9543851
CAGAGTC7400.00226103758.7894053
CTCAATT9301.8973605E-48.6494871
CTCATAC10406.856529E-58.4377861
CTCAAAT11201.6245838E-47.83508731
CTTCAGA14951.2747096E-67.8263511
CTTTAAG13101.4713763E-57.81515031
CTCTGGT9650.00259736047.57797671
CCCTCAT9700.00273227657.5389151
CTCCAAT9900.00333610747.3866141
CTCAAGG12003.601872E-47.31274751
CTCGAAG11000.00114994547.31274751
CCTCGAT17201.3423487E-67.2277161
CTCAAAG12355.008602E-47.1055041
CCCTGAT14857.725286E-56.8941731
CTTCAAT18052.867364E-66.88735251
CTTCGGT11750.0023104876.8459771
CTCAAAC11850.00252605446.78820471
Produced by FastQC (version 0.11.5)