FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028053
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTA4050.002441311512.46468454
CTCCAAT11601.4551915E-1112.0389481
CCTTAAC4800.00753039910.7188871
TCCAATC10250.00535590067.0358152
CCCAGAA10500.00562699937.00008961
CTTTGAT17451.1873424E-56.73934131
CTTTATT20051.3568071E-56.23200041
CATCAAT22652.1424312E-66.1656421
CTAAAAA13450.0089657676.01122951
CTTTAAT13450.0089657676.01122951
CTTCCAT21101.8839924E-45.5735311
CGATTTC17850.00610207255.2522264
Produced by FastQC (version 0.11.5)