FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054044
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT10506.5865606E-810.4506591
CTTTAAT31250.010.300171
CTTAACG7752.9389188E-49.4393041
CTACTGT8851.1031612E-49.0926641
CCTCTAA6500.00702350639.0036451
GTTAGAT6650.0084680868.8005551
CTCACTT9402.1229695E-48.5606471
CTTAAAT19905.2750693E-118.4550561
CTCACCT10507.6393626E-58.3605271
CCCATTA9652.8193346E-48.3388681
CTCATAT16751.1956217E-88.2981361
CCCCCTC9050.0013766818.0833831
CTTAACA13853.6042184E-67.92288261
CCCCTCT8550.00745130477.70048571
CTCATTT21351.9354047E-97.5381811
CCTAAAT9750.00286420717.5030381
CTCCAAT18557.295603E-87.4929251
CCCCTTT17702.652523E-77.4394521
CTACAGA10859.903345E-47.4165971
CTTCCTT39750.07.36147071
Produced by FastQC (version 0.11.5)