FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254130
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACG6000.0040051279.6387787
CTATTGT8100.00498508158.0528661
GTTTAAT10200.00486874257.105471
CTATGAC10600.0070702826.8373391
AGATATA15802.009319E-46.40679454
CTGAAAT20801.8782995E-45.5750611
CTTTAAA19150.00227108325.29849151
CTTTGAA24152.8250052E-45.10181571