FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542798
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT6707.417085E-510.81773951
CTTACTA6950.00124370929.3857511
CTCCCTC8105.0165906E-48.9480061
CTCAACG6650.009134348.719261
CTTGAAT15804.962203E-98.7158121
CTCCCAC9302.0898394E-48.5727671
CTTCAAT28550.08.1237251
CTCAAAG13502.8546729E-68.05320551
TTCTCAG10155.578513E-47.82582576
CCTTGAT19653.6907295E-97.74583151
CTTAAAT14507.872721E-67.4978121
CTTATAT22508.60382E-107.408951
CTTTGAA27603.6379788E-127.35292671
CTCAATT11150.00145944117.150381
CTCAAAT14305.2902964E-57.09583141
CTTCACT13602.1989856E-46.9281261
CCTTTGG15702.4071138E-56.9247311
CCCGAAT10500.00644979356.90274761
CTTCAAG20203.802852E-76.8173181
CTTAAAG12909.1143936E-46.7422191
Produced by FastQC (version 0.11.5)