FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434236
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1652.5920053E-630.4266197
CACGTCT2208.2091356E-726.0799629
GCACACG3106.9095586E-720.8219016
ACACGTC2104.6349553E-420.4913968
AGCACAC2550.001708350116.8752675
GAGCACA2600.001945249216.5507434
TATACCA2700.002502890315.9377523
GTTTGAT4700.00741056910.7398121
AAGGAGC5500.002074904510.4319849
ATCAAAT6305.2779546E-410.3014531
CGAAAAG9600.0028219227.51147561
CAAAAAG10600.007401496.80284551
Produced by FastQC (version 0.11.5)