FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796796
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAG7403.7289283E-1014.7984931
CTCAGAT4805.7353446E-412.167651
CTCAAAT7601.6846589E-611.5272481
CATAGAT7151.3298957E-410.2106161
CTTAAAT6250.00516749279.3447561
CTTGAAT8709.342212E-59.2306311
CCCTGAA6550.00759650678.9167521
CTCCAAT6600.00808518.84921
CTCTTAT6650.0086006788.7826641
CTTTAAT10054.6740497E-58.7171231
CTTTGAA13101.6696904E-68.3594551
CTTTAAA9703.0409818E-48.279021
CTTTGTT11602.7902439E-58.1816961
CTCCATT9000.00132759548.1117671
TCAGCGG8200.00575592567.92432554
CTTCCGT9300.00183443997.85009671
CTTCGAT8550.0075778967.6848321
CTTCGGA8750.0093167177.50917861
CTTGGAA9950.0035575137.3372771
GATATTA11450.00201389786.9348825
Produced by FastQC (version 0.11.5)