FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539983
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT7251.5706544E-410.0451891
TCGAATT9753.6983747E-69.6018852
CCCCAAC6950.00119032269.4309141
CTACCAT9452.3356966E-59.24795151
CTCATAC10951.3969802E-58.6462021
ATAACGT7600.00268540258.6243231
CTTTAAT20501.2732926E-118.526161
CTTTAAC12105.3677777E-68.4263361
CTTTGAA24251.8189894E-128.1086431
CTCACCT9200.00169159177.9160461
CTCAGCT9400.00209027587.74761871
CTCTGAT12305.9910108E-57.6972281
CTCAGCG11402.0913397E-47.6660651
TTTAACA17502.8495924E-77.4071692
CTTTAAA21702.9394869E-97.38344571
CTTTAAG15151.3650817E-57.2106561
CCTTGGT12254.7838603E-47.13413431
CTCGAAA10400.00561093037.00265551
CTTAATT11500.0019234626.96612071
CTCCGAA11500.0019234626.96612071
Produced by FastQC (version 0.11.5)