FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627209
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT6350.006193569.1423561
CTCTGAT15101.9101572E-78.1698461
CACAAGA13452.6611542E-68.0930241
CATCGAA14601.001561E-67.9525981
CTTGACC10900.00113266467.3233211
CATCGGG10200.0048111017.11445571
GTTATGT10500.00637434846.91118571
CCTCGAT13200.00116688046.59704071
CAATCCA14454.836127E-46.49505334
CTTTGAT32853.6379788E-116.40625861
CATCGAG12600.00520552146.33525371
CCTGGAT16653.7763885E-46.10176751
CTTTAAT27001.4973011E-65.6441351
ATATTAG15750.0088998545.49972776
CTTTCAT32402.5600457E-75.37536671
CTTCAAT26203.0966432E-55.26252461
GTTATAT18400.0080988355.1270481
CAACAAG19850.00350498315.1181071
CATGGAT21350.00156678865.09841541
CTTTCAC20100.004096015.0544491
Produced by FastQC (version 0.11.5)