FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463630
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1753.3669494E-936.0531357
ACACGTC2355.9402737E-826.848088
AGAGCAC2501.0816984E-725.2371943
CACGTCT2752.716497E-722.9429059
GAGCACA3006.2787876E-721.0309944
AGCACAC3108.603456E-720.3525775
GCACACG3603.5979538E-617.525836
CTTCGAA2902.3279605E-416.996651
GAAGAGC6354.287065E-612.1978211
AAGAGCA5954.018836E-410.610732
CACCCTA1658.2976336E-48.50746525-29
CTTTTAT7600.0036322198.3385811
GGATGAT8100.0064436567.82385441
GGTGGAA8150.00680901257.7758551
CCCTACT2355.3733576E-57.765324625-29
AAGATTT8350.0087998137.55604653
ACCCTAC1900.00333161847.38806225-29
GGCAAAG9650.0037474817.2968591
CCTACTG2550.00115009516.605796325-29
Produced by FastQC (version 0.11.5)