FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861385
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT4650.006718624410.8844421
CTCTCAT5950.00372998539.72151851
CTCCTAT6050.00428048159.5608321
AAGTTAT8206.1197707E-48.7704475
CATAGAT7500.00254032928.67645551
CTTCGTT8900.00130822888.12402251
CTTCAAC11854.112404E-57.93206171
CCATTGA9250.00191363937.81662651
TCGTTTC9300.00212448937.733983
CATCGTT9800.0033713137.37793831
CCTTGAT16152.6654065E-46.2678211
CTTTTAT15259.098874E-46.1636021
CTTCCAG15800.00139285395.94904661
CTTCAAG14650.00391446665.9224951
Produced by FastQC (version 0.11.5)