FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668075
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAG1600.002153962622.6930451
CTCGCGG2950.004158190514.7697091
CTCCCAG4500.00509449911.2960921
CTTCGTT7001.1320047E-410.3739621
CCTCCTT8608.917606E-48.4439231
AATGTTA8000.00486894148.0728885
CTCAAAA9550.00250732227.60395151
CTTCCAG11200.0014963117.1320991
AAGGATT10100.00486222757.10538343
CTTTGTT10550.0066175536.8831981
CTCTTTT16103.3166158E-56.7656281
TGGATTT13600.00184900486.33215053
TAGATTC3059.991461E-46.11734470-74
AGATTTT16150.00197724045.77669864
Produced by FastQC (version 0.11.5)