FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545069
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAT4754.8827095E-412.4011621
ATTTGCG4550.0057326311.1179766
CCCGGCT6959.139516E-510.5945181
CAATAAT9151.2290548E-610.4613621
GATTTGC5800.00302938479.967845
ATTTGAT8500.0066540087.7963181
CATCAAA10900.0079286926.75521951
CTTCTTT15751.5162758E-46.5450581
CTTTTTT17650.0041747255.42331271
Produced by FastQC (version 0.11.5)