FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962020
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCT7851.4695615E-812.9442011
CTCAGAA6806.5844742E-611.7408791
CCCACTT5750.002723548310.0980841
CTAAAGA6200.0050771269.3651591
CTTCAAT16401.0059011E-98.8512171
TCAATCT13955.1174593E-67.7284273
CAACAGT8500.00757986037.6849391
CTTTGTT16659.0235517E-77.4105681
CTTTAAA18659.174801E-87.39420751
CTAAAAA14254.952018E-57.1306651
CCAATAT11750.00249853126.79472161
CAATCAT12858.573677E-46.77789741
CATTAAT10850.00872921856.6893991
CTTTCAC11000.0099639496.598181
CATAAAT16705.5654848E-56.519161
TTAATAT17802.2113802E-56.4606173
TCAATTT17751.4751048E-46.07389072
CTTTTAA17406.6190073E-45.8397691
CAATCTT14950.00524131445.76918034
GGAAACA15300.00677154545.63720562
Produced by FastQC (version 0.11.5)