FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464406
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACT1952.626775E-422.2888491
GAATAAG3101.5150288E-518.6354486
CTTGAAT5208.5219806E-512.5374771
CTATGAT4200.003096793812.0731261
CCAATGT4600.006250973310.9888383
CTCAAAA4650.006618344710.9047581
CTTTGAA6100.00450468859.5001651
CTTTGAT6200.00515079829.3469361
CCTCCTT7250.00183245978.9923971
CCATTTT7400.0022710038.7825853
TGAATTT7600.00289203938.5514643
CTCTTTC8000.0044675068.149361
Produced by FastQC (version 0.11.5)