FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777920
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAAAC1606.272909E-428.040092145
TTAAACT4300.003829626611.7368243
CTCCGTT6807.4967305E-510.8047521
CTCCGAT6200.00458890679.4802991
CTCATAT6450.00635388269.1128451
CTCCACA7800.0031330288.4775751
CTTAAAA9602.5382516E-48.4187021
CTTCAAT11452.1877035E-58.3418351
CCTTCCA8250.00519523358.0151621
CTTGTTT10506.6508667E-47.69709971
TCAAAGA13000.0076864846.10096172
CTCTTTT18151.4793791E-46.07209251
GATATTC14450.00346571025.98734665
CTTTATT15300.00530478545.76253461
ATATTCT17950.00107004325.6232146
Produced by FastQC (version 0.11.5)