FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857342
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG7055.0156814E-813.3882261
CTATGAG6203.301697E-511.7105451
CTTCGAA5601.6683164E-411.6687211
CTCAGAA6204.2846697E-410.539491
CGAATCT4900.00960727510.3721961
CCCATAC5750.002715167810.1016171
CTCAACA5900.00336081819.8447971
CTCCTAT6300.0057742089.219731
CTCACCG7250.00179779059.0130821
CCACGAT7250.00179779059.0130821
CTTCGCT6450.0070058689.0053181
CTCATTT9703.288609E-58.9821081
CTTCAAT14005.579932E-88.8163671
CTCTTTT24200.08.4006221
CTTTAAT12105.0654126E-57.80057761
CTCTCTT8400.0067951187.7791471
CTTATTT16155.526308E-77.64267161
CACAGAT11700.00237938276.82614661
CAACAAC10750.00795416.75398831
CCTTTAT14103.3422088E-46.6941131
Produced by FastQC (version 0.11.5)