FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849532
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT3254.0340747E-415.8164191
GGTTAAG3250.007341494313.556931
CTCAGAT4050.002130692112.6921881
CTTGAGT4450.00501877111.319423
CGAGAAT6053.0765394E-410.9239731
TACCTGA5650.002533033710.186519
ATTAAGG6050.0044525369.5152637
CGTTCAT7100.00133990969.3084551
ATATCGC9000.001531337.9954646
CTTTCAG9950.00336081727.38023851
CTTTAAT16052.721519E-56.86293171
AACCATT10500.0069076176.85325432
AAACCAT10850.00779538146.7680531
AATATCG12400.00480352066.3834755
CTTCCAT14100.00214469746.2496491
CAACAAT13500.0094002235.983461
TCCATTT17407.382743E-45.78981833
CTTCTTT30101.5467176E-65.36722331
Produced by FastQC (version 0.11.5)