FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292942
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT7501.828837E-510.710191
CCCAAAT13903.8198777E-1110.5070521
CCCGAAC10751.0436925E-69.5101051
CTTAAAT19653.6379788E-128.9189631
CTTAGAT8255.5931066E-48.8513961
CCCACTT12901.3397075E-68.4911641
CTCCCAT18851.4224497E-98.1353011
CTCAGGT11051.4135224E-47.93020961
CTCCGCT14305.8228197E-67.65986161
CTCGCAA12557.444872E-57.5642411
CCCAGCT20851.0075382E-87.3549361
CTCCGTT17652.0651441E-67.03347441
CTCAAGG20908.165807E-86.98794361
CTTCGAT25109.895302E-106.9823761
TCGATCC10550.0069663286.8483473
CTTCAAT35300.06.8266071
CAACGTA10700.00720354736.82467461
CTCATGG19301.1511074E-66.81052971
CTTTTAT42050.06.7727391
CTTTTGT36900.06.72849941
Produced by FastQC (version 0.11.5)