FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286218
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT7056.3149855E-813.1575719
CACACGT7602.7326456E-510.3276267
ACACGTC8750.00126223098.1548018
CTCCGAA17854.449539E-77.2124491
CCCCGGT13301.9695907E-46.9910151
CTCAATT15702.8999148E-56.83345131
CCTCAAT16556.033868E-56.48248861
TCGAATT14850.00526408255.7677872
GGTTGAT21202.9824112E-45.3979971
CCTTTGG24105.86096E-55.3420011
CTTCGAT21904.7280756E-45.22545861
CTCCAGA21100.00162532315.0846061
Produced by FastQC (version 0.11.5)