FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770623
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC4100.002460282512.4511481
CTCAGAA5451.2427551E-412.0431811
CCTTAAT4500.004936231311.3443791
GTCAATT5750.002616524710.1465251
CACGATC5950.00384643029.6851972
CCCAGAA6200.00487892249.4100851
CTCAAAT7150.00152103539.1797681
CTCAACA6400.00633569679.1160191
CTTCAAA13854.357753E-88.951471
CAACAAC10801.0971732E-48.1031281
CTTCTGA9954.0452596E-48.0624081
AAGATGT9904.375762E-48.0037383
CTTATTG8250.0055540787.95579861
TAATCCA8250.0062048437.8582164
TCAGAAT12005.0384064E-57.8036452
CTTGTAT8450.0068852457.76749561
CTAAAAA10708.8069163E-47.4972871
TACCTTT10250.0054127917.02767322
CTTCTCT11000.0095100626.62983231
CTTCTAT11000.0095100626.62983231
Produced by FastQC (version 0.11.5)