FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704715
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT15752.5314395E-56.9004421
TACGAGA13200.00120527016.57862769
CTCGAAA14705.706185E-46.4075531
CAAACCT14850.0044575485.85492131
CCACAGA22401.4839357E-55.82224851
CCCACTG15250.0059861645.7013491
CTTCCGA22551.0184124E-45.46221261
CACCTAT15950.009818085.45113331
CTCCGAT20257.9105125E-45.3670111
GTTTAAA26553.96517E-55.18507771
CTTCGAT29507.809374E-65.15778831
CTTAATT20050.00405216645.0591771
CTTCACT23007.861592E-45.0403231
TATTCGG20250.00465503755.00300557
Produced by FastQC (version 0.11.5)