FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405830
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC11300.018.4989858
CACGTCT11600.017.3991649
CACACGT12050.016.7494037
AGAGCAC17100.012.6475873
AGCACAC16350.011.9034915
GCACACG16550.011.7596436
GAGCACA21400.09.4324994
GAAGAGC34151.8189894E-126.5782011
AAGAGCA38350.06.39272932
GTTCGAT16500.00228095055.70946651
CCCAAAT15500.0071510715.6102951
AAGTCAA23401.9176373E-45.23674445
GTTCTAT21200.00145466295.1273331
AGATTGT28302.2771108E-55.0941526
Produced by FastQC (version 0.11.5)