FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688813
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT3554.83463E-516.2824731
CTCAATT4403.0037714E-413.1369971
TGCTCCC4654.916705E-412.3919049
CTCTGAT4907.4385985E-411.7964861
CTTAAAG5852.616867E-411.115921
CTCAATA5650.002442853510.230581
CTTCGGA6550.0082674348.8248521
CATCCAC7900.00408703288.2314091
CTTAAAA9904.2330837E-48.0281651
CTTTAAA10054.974844E-47.9083411
CTTTTGT12205.9557427E-57.6991411
CTTCCAT12256.2664665E-57.6677161
CTTTGAT10400.0060485936.9474491
CATCAAT11950.0031248316.65094761
CAATTTT12150.0038187426.52483843
CTTTTAT13450.00151155566.4464071
CTTTATT14950.00493844465.799611
CTTTCTT18050.00111646825.6041481
CTTCTTT21052.3210846E-45.49195051
Produced by FastQC (version 0.11.5)