FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905149
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4050.039.488127
CACGTCT4350.038.3632749
ACACGTC4950.032.3084648
AGAGCAC6300.026.481613
GCACACG7250.022.0576636
GAGCACA7400.021.6105484
AGCACAC8350.019.9845545
GAAGAGC10050.017.323681
AAGAGCA13100.013.8005292
CCCACTC3100.007627720513.4789411
CTCGTAT3350.09.96024735-39
ACTCCAG4750.09.9507415-19
AGTCACT3950.09.50373620-24
CTAAAAA6600.00111628999.4965271
GTCACTC4150.09.04572520-24
CTTCGAT8600.0013508448.0978141
ACGTCTG5951.8189894E-127.0097610-14
TCTCGTA4859.858923E-96.59310235-39
TCACTCC5956.184564E-116.542226325-29
CAGTCAC6202.5465852E-116.50330320-24
Produced by FastQC (version 0.11.5)