FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902178
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3502.3016491E-618.3695957
CACGTCT4252.468255E-413.4462799
TCGCCTA4050.002605432612.3567782
CCAGGAT6103.1254975E-511.7736831
AGAGCAC5050.001052502111.3186853
GCACACG6455.831306E-511.0755536
ACACGTC6003.6789494E-410.7150038
AGCACAC5500.002147991410.39088255
GAGCACA6558.233256E-49.8174464
GTTCAAA9900.0039752737.25449041
CCTGAAC10000.00438422857.1819461
CTTTGAT11800.002917966.69503451
AGTCACA3508.4901677E-45.72825620-24
Produced by FastQC (version 0.11.5)