FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565723
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGACTTCGATCTCGTATGCCGT29190.5159769003558279TruSeq Adapter, Index 4 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5300.083.240337
ACACGTC5800.076.0644458
CACGTCT5800.076.0644459
GCACACG7000.064.00016
AGCACAC7450.060.134325
AGAGCAC7900.059.2813843
GAGCACA7950.058.059934
GAAGAGC9850.045.6275141
AAGAGCA11500.041.3212362
AGTCACT4800.018.94617520-24
TCGAGAG2250.001069015218.0994387
ACTGACT5300.016.90299625-29
ACGTCTG6000.015.6096610-14
CAGTCAC5750.015.57987720-24
CCAGTCA5950.015.28430820-24
CTGACTT6000.014.9309825-29
GTCACTG6400.014.84588220-24
CTCGTAT6000.014.70475335-39
ACTTCGA6200.014.66930330-34
TCTCGTA6200.013.57361835-39
Produced by FastQC (version 0.11.5)