FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841359
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAA9001.13761416E-711.1731221
CAAACGA6003.4960563E-410.7740831
CCACGAT7703.304297E-49.3282111
TTTACTG7703.435202E-49.2921327
CAACCAT7753.525742E-49.2680291
GTCCATT6350.00673217569.0490991
GTACATT6350.0069464569.0146362
CTTCGTT7550.00286437018.5621851
CTCAAAA9703.6245972E-48.1453551
GTTCAAT8050.0051084828.0303731
TCAAAAC9700.0033768287.3766682
CTTTTAC9700.00337879227.3762295
CTTTGAT12851.2204721E-47.2665671
GATGATC11350.00201472736.9347172
TTAATTT10550.00764275346.7819353
CTTTTAT13400.0015505926.43228861
ATCAAAA17159.27474E-56.2822641
CTTCATT14300.00321753186.0274591
TAAATAT14350.0034933715.98322633
CTTCAAA13250.0097383755.96301461
Produced by FastQC (version 0.11.5)