FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512009
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGACGAATGATCTCGTATGCCGT5970.11659951289918731TruSeq Adapter, Index 6 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1750.065.385497
ACACGTC2000.064.3638468
CACGTCT2050.059.305449
AGCACAC4150.032.7387855
GAGCACA4150.031.0156924
GCACACG4100.027.9084426
AGAGCAC4600.026.4295983
GAAGAGC8150.017.6082571
AAGAGCA9050.015.0172132
GCCTCGA3000.005031907514.350731
TTGTCCC3300.00966585213.0027973
AGTCACG2207.2759576E-1212.39332620-24
CTCGTAT2951.7880666E-99.22530635-39
CAGTCAC3002.3428584E-99.0884420-24
ACGTCTG3053.3487595E-98.90976510-14
GTCACGA3452.8487193E-87.90299120-24
CGAATGA3707.9458187E-76.96505630-34
CGTCTGA4055.01901E-76.709823610-14
CTCCAGT4106.208502E-76.627866315-19
TCGTATG4301.3673107E-66.330970340-44
Produced by FastQC (version 0.11.5)