FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665105
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3002.6942871E-823.1469359
ACACGTC3454.8712536E-922.1405458
CACACGT3358.661664E-820.7285967
AGAGCAC3652.1355845E-719.0277373
GAGCACA4701.6842387E-716.2545474
GCACACG4703.0024112E-614.776866
AGCACAC5157.737863E-613.4856785
GATTGTG6200.0072887598.9614515
AATGTTA7000.0019557088.9294445
AAGAGCA8952.1829236E-48.5365492
GAAGAGC8350.00101880058.3312951
AGTCACG2101.3881236E-47.942898320-24
TCTCGTA4150.00191639927.0295405145
TCACGTA2805.2574894E-46.451080325-29
CACGTAT3602.846905E-45.78943125-29
AAAAAGC18303.5597812E-45.69272476
GTCACGT2950.00656238345.65426720-24
CAGTCAC3050.0094754975.46888120-24
ACTCCAG3650.0021270445.32696615-19
TCGTATG4203.94755E-45.29271740-44
Produced by FastQC (version 0.11.5)