FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523896
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT4901.6601552E-716.2734031
CTTCGAA3153.5055296E-416.1090261
CTTTCGA4352.6505085E-413.3316071
CTCAAGG4654.6551612E-412.4715041
AGTATTA4750.00790116710.6481525
CTTGAAC4800.00833584710.5715481
CTTCTAT5600.002206544410.3558031
CTCCATG6557.1983767E-49.9605421
CCTCAAA5900.00340132889.8292351
CTTCGAT6100.00447980739.5069661
CTATAAA6200.0051223849.3536281
CCATCAG6500.0077514248.8938113
CTCAAAA8306.3697493E-48.7338091
CTTCATT9402.3343245E-48.4829441
TTCCGAT7750.0034400938.38933859
CTCTTTT10701.05407606E-48.1297881
GATTTAT8100.00511347468.0283695
GAATTTG8100.00511347468.0283695
CTTTTAA9100.00158558277.96600151
CTTTTCC16901.159031E-67.29195551
Produced by FastQC (version 0.11.5)