FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436883
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAG2900.003725141315.0143581
CTGACAT2900.003725141315.0143581
CTTGAAT4352.6246734E-413.3460951
CCCAAAG4000.002118490612.6996431
CAACGAT4200.003054783412.0948991
TGAAGTA4806.205431E-412.0519414
CTTCCAT7201.5124782E-410.07908251
AGAATTT8800.00116582098.2172324
CTCTTTT8350.00645696147.8218511