FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350926
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTA950.00449237411.490701135-139
CCCACGA6550.0091897448.7126751
CTCCGAA11904.3497377E-47.1934491
CTCAAAT11300.00198834166.9441181
ATTTAAG10250.0061968226.93118056
CTCGATG12600.0062188256.2276611
CCTTCAT19601.0045375E-45.8232681
GTTTCAT18452.7746696E-45.7995961
TCCGAAA18803.7649833E-45.67055132
CTTTAAT17550.00568851885.28407671
CTTGAAA22557.400377E-45.06146571