FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818314
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT12601.1054126E-68.6052891
CTTACTT11904.745898E-68.5040511
CTTAGAA9800.00338454997.37596271
CCCAGCT13853.5962585E-57.306731
CCCAGTT12000.00325728076.62607341
CTCAAAT16756.151342E-56.47323271
CCCAAAT12300.0042177196.4644621
ATCGAAT16303.0209514E-46.20847851
CTTCGAT18352.1541485E-45.9088091
CTCATAT19951.0725547E-45.7972481
CTTTTCT42805.456968E-125.74222141
CTTCGTT17708.686952E-45.71741251
CCCGAAG17759.00445E-45.70130731
CAACAAT24359.7289085E-65.6402641
CTCCAGA16950.00323268685.54393861
CTTCTAC18300.0013261775.5299571
CTTGCAT17400.0044048845.40056131
GTTGATA23001.4439624E-45.3365912
CTCAAAG17750.0055670925.2940711
CTTCACC25008.8413995E-55.2044791
Produced by FastQC (version 0.11.5)