FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178358
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT6200.026.4617449
ACACGTC6800.026.2248748
CACACGT6350.025.8366647
AGCACAC7150.023.9434935
AGAGCAC7600.023.462373
GAGCACA7550.020.7871484
GCACACG7550.020.7853816
AAGAGCA14800.011.0876772
GAAGAGC16059.094947E-129.8004261
CAAACGA7450.00264783838.6374081
TCAAGAT13050.00124571386.55867623
AGTCACG5303.6099664E-86.19005120-24
CTCGTAT6305.6206773E-95.88694335-39
CAGTCAC7053.43789E-105.867473620-24
CAAAAGA18200.00141929825.4998891
ACGTCTG8051.6916601E-95.316148810-14
GTCACGT6453.3452852E-75.30754620-24
CCAGTCA8152.3228495E-95.250563620-24
Produced by FastQC (version 0.11.5)