FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058755
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10851.6982811E-49.6563635145
CCCTAAT6609.785659E-49.6356731
CACGTCT12409.786702E-57.39935169
CCCAAAT19052.13764E-77.0476131
CTCCGCT23652.0842708E-76.2743911
CCCAGAT23902.5399822E-76.20875931
CTTTGAT43650.06.1515251
CATGCAT14750.0054623765.74873971
CCCAGAA27254.8253605E-75.7047891
CTCAAAT21105.4425444E-55.69311481
CTCCCAC17409.3375123E-45.6854161
CCTCAAG18904.3811207E-45.60806371
CCCAAGG16500.00307799065.5672781
CTTTAAA38607.3305273E-105.4918341
CCTCTAT22301.2560264E-45.38675831
CTACAAT28456.3760162E-65.21579461
CCCTGAC19100.00302426635.17938381
CTCCCGT19150.00312466695.16586071
CTTCAAA54603.6379788E-125.0472571
GTCAGGG22407.7128294E-45.0472571
Produced by FastQC (version 0.11.5)