FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474948
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT2750.00262472815.8250291
CTCATAG4856.6014857E-411.963941
CTCAAGA4300.003658079311.8074331
CTCAACA5008.52456E-411.6050211
CATAGAT4800.00829923610.5774931
CTCATTA4850.0089619710.4684471
CTCATTT6500.00751645548.9269391
CTTTAAT7550.00261777978.6461251
CAAAGAA13200.0084492656.04428151
Produced by FastQC (version 0.11.5)