FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660080
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTT2600.001638271316.983051
CCCACGG4300.003283464111.980291
CCCATGA5350.001330778211.0045921
CCCAAAA5500.00167602410.7044681
CTTCAAA12204.7293724E-910.2547921
GTTAAAT5800.002606524410.1507881
CAATACA6450.00626646779.127841
CTTTTGT9552.3552627E-48.4767051
CTTCAAT9000.00122577288.1770241
CTTTGAA11953.657763E-58.0059561
CCTGCTT11101.3566209E-47.9560231
CTTATTT10650.0063712686.9101611