FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748780
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAG6150.00478607979.4332921
CTTAGAT6250.0054661279.282361
CTCCACT8851.2129473E-49.0135911
CGTAGAT8155.306278E-48.8979671
CTCAAAT17303.0195224E-108.8028161
CCCCCTT7450.00232987088.7606171
CTTTAGT7850.0037400758.3142151
CCCCGGT8900.00127209228.1481391
CTACGAC9100.00158417097.96905851
CTTCAAT24751.8189894E-127.91110231
CTCTAAT8250.0058498987.9111021
CTCAAAC13051.5639598E-57.77975561
CTTACGA9900.00362099387.3250951
CTTACAT11000.00125714367.2518441
CTTAAAG20004.1338353E-87.2518431
CTTTAAT27652.5465852E-117.0813661
CTCATTT21652.372144E-87.0341211
CTCCAAA20657.4325726E-87.02357771
CCTCCAT19009.958658E-76.87016731
CCCTTCT11700.00241314086.81797271
Produced by FastQC (version 0.11.5)