FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076886
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT4001.3093153E-414.4896981
TTCCCTA4453.3023595E-412.9917938
TCCCTAC3350.00997207212.94331559
CACTCTT6556.0576065E-511.0346422
CTCATTT9300.001979567.790161
CTCAAAA14606.981245E-56.94711541
CTTTAAA16403.1701988E-46.18462751
GTTTGAA13100.0079251976.08346131
CAATAAT16700.00263552765.63970331