FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411518
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6200.0047642079.4382931
CTCCCTT9552.5111216E-59.1912171
CTCAAGA10951.321743E-58.6840921
CTCCAAG9050.00137746978.0825161
CTTCTCT18901.01361366E-77.35337921
CTCATAA13201.3604795E-47.20384841
CTCAAAT11300.00152540537.12048241
CTTCGAT13601.9644061E-46.9919711
CTTCAAC17952.6169582E-66.9275491
CTCCGAT10600.00646800736.9006391
CTCCTGT13059.36319E-46.726141
CACATTG12200.00341926636.5952011
CTTTGTT24653.436071E-86.5283121
CTTATCT17207.526781E-56.3790791
CTTTGGA19509.523163E-66.3768981
AACCAGA13000.00760870336.10776766
CAATAAG14400.00284144426.0955641
CTATAAT17305.573137E-45.9193921
TTCGATA17206.1268895E-45.87575342
CTTCAAT23855.6062363E-65.8272061
Produced by FastQC (version 0.11.5)